Figure1. Examples of correlation between RCI and other methods of measuring motional amplitudes in proteins.

NMR RMSF - root mean square fluctuations of atomic coordinates in NMR ensembles, MD RMSF - root mean square fluctuations of atomic coordinates in MD ensembles, S2 - model-free order parameter, RCI - random coil index, B-factor - temprature factor of X-ray structures; RCI->NMR RMSF - root mean square fluctuations of atomic coordinates in NMR ensembles predicted from RCI, RCI->MD RMSF - root mean square fluctuations of atomic coordinates in MD ensembles predicted from RCI, RCI->S2 - model-free order parameter predicted from RCI, RCI->B-factor - temprature factor of X-ray structures predicted from RCI.